Proteomics Human Diseases and Protein Functions Part 7

Tham khảo tài liệu 'proteomics human diseases and protein functions part 7', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Proteomic Approaches to Unraveling the RB E2F Regulatory Pathway 139 E2F member Interacting Protein Function Reference JAB1 Promotes apoptosis Hallstron Nevins 2006 EAPP Activates transcription of TK and represses transcription of p14ARF. Promotes proliferation Novy et al. 2005 E2F1 SKIP CBP TEF-5 B-MYB MGA . Giangrande et al. 2003 MCRS1 E1F1 FHOD1 FLNA SUI1 A-FABP MX1 RNF2 PRMT3 RANBP9 . Hallstron Nevins 2006 E2F2 RYBP B-MYB Activates transcription of CDC6 . Schlisio et al. 2002 Giangrande et al. 2003 ALIEN EAPP . . Escher et al. 2007 Novy et al. 2005 RYBP Activates transcription of CDC6 Schlisio et al. 2002 TFE3 Activates the p68 subunit gene of DNA Pola Giangrande et al. 2003 E2F3 RXR-BP WNK1 PKI-B SKIP RYBP CBP TEF-5 B-MYB MGA SPIB . Giangrande et al. 2003 HSP86 A-FABP MX1 RNF2 PRMT3 RANBP9 . Hallstron Nevins 2006 ALIEN . Escher et al. 2007 EAPP . Novy et al. 2005 E2F4 ALIEN MGA . . Escher et al. 2007 Giangrande et al. 2003 E2F5 ALIEN . Escher et al. 2007 E2F6 ALIEN . Escher et al. 2007 Table 1. E2F-specific binding partners identified through proteomic approaches. . not determined these interactions it has been proposed that transcriptional regulation of specific E2F target genes can only be achieved when relevant interacting proteins act jointly forming a functional complex on the promoter Freedman et al. 2009 . Therefore the unique marked box of each individual E2F factor appears to play a pivotal role in determining the specificity of interaction. Other proteins exhibit E2F-binding activity independently of the marked box allowing the mapping of distinct functional domains within the E2Fs. A yeast two-hybrid interaction 140 Proteomics - Human Diseases and Protein Functions screen with the amino-terminal region of E2F1 comprising the NLS but lacking the marked box led to the cloning of EAPP E2F-associated phosphoprotein Novy et al. 2005 . EAPP appears to potentiate the proliferative functions of E2F1 in several ways.