Project Number Project Title Vietnamese Institution Australian Institution Commencement Date VIE36 IF03 Moulting and growth of mud crab (Scylla paramamosain) larvae treated with extracts of neem tree (Azdirachta indica) and Cell Salts Research Institute for Aquaculture No. 2 Charles Darwin University May 2006 Completion Date December 2006 Objectives: To evaluate the effects of neem extracts and cell salts as an antibiotic replacement on mud crab (S. paramamosain) larvae in terms of moulting, growth, and survival rate from Zoea 1 to Megalopa. The effects of neem extracts and cell salts on the bacterial and fungal communities of the culture medium will also be. | Project Number VIE36 IF03 Project Title Moulting and growth of mud crab Scylla paramamosain larvae treated with extracts of neem tree Azdirachta indica and Cell Salts Vietnamese Institution Research Institute for Aquaculture No. 2 Australian Institution Charles Darwin University Commencement Date May 2006 Completion Date December 2006 Objectives To evaluate the effects of neem extracts and cell salts as an antibiotic replacement on mud crab S. paramamosain larvae in terms of moulting growth and survival rate from Zoea 1 to Megalopa. The effects of neem extracts and cell salts on the bacterial and fungal communities of the culture medium will also be monitored. Activities 1. Produce Zoea 1 of the mud crab Scylla paramamosain produced by spawning of the broodstock housed in the hatchery at Bac Lieu RSA. 2. Conduct Experiment 1 to determine the effects of neem extract on larvae Z1 s moulting and growth and water quality. 3. Conduct Experiment 2 to determine the effects of cell salts on larvae Z1 s moulting growth and stamina. Milestones Expected Date Nb r Milestone Description Deliverables 1 CARD Contract Signed Research Agreement signed. Milestones and payment schedules in place April 2006 2 Research Report A summary report of all trial results including the water quality and survival and growth rates of mud crab from Zoea 1 to Megalopa stage and analysis of economic and environmental benefits. December 2006 PROJECT COMPLETION REPORT 1. Introduction In Mekong delta mangrove forest distributes along the coastline of 700 km and it is a homeland for mud crab in tropical areas. The mud crab becomes potential to be alternative species from failed shrimp farming. Particularly mud crab hatchery has been introduced and operated recently at Bac lieu Tra vinh Soc Trang Ben Tre Kien giang and Ca mau provinces. However the survival from zoea stage to crablet stage 1 is low at approximately 5 . In addition like shrimp rearing the mud crab hatchery is using antibiotic as major .