Periparturient period is especially critical for health and subsequent productivity of buffaloes. The present study was undertaken to assess the degree of oxidative stress in Mehsana buffaloes during periparturient period. The results of the present study revealed that the antioxidant markers . Melendealdehide (MDA), Super oxide dismutase (SOD) and Glutathione peroxidase (GSH-Px) were significantly (p | PRODUCTION SYSTEM 09 PRODUCTION SYSTEM Antioxidant Status in Periparturient Mehsana Buffaloes Pathan Latif A. 2 Das Siddiquee G. M 4 and Vadodaria V. 1Ph. D. scholar Animal physiology NDRI Karnal Haryana. 2Professor Head Dept. of Animal physiology Biochemistry SDAU Gujarat. 3Assistant professor Dept. of Animal physiology Biochemistry SDAU Gujarat. 4Professor Head Dept. of Clinics SDAU Principal College of Veterinary Science A. H. SDAU Gujarat India ABSTRACT Periparturient period is especially critical for health and subsequent productivity of buffaloes. The present study was undertaken to assess the degree of oxidative stress in Mehsana buffaloes during periparturient period. The results of the present study revealed that the antioxidant markers . Melendealdehide MDA Super oxide dismutase SOD and Glutathione peroxidase GSH-Px were significantly p increased from 30 days before parturition attaining highest levels on the day of parturition. Significantly p decreased levels of these marker enzymes were observed from 15 days after parturition to 30 days after parturition. This indicates higher oxidative stress and lower anti-oxidant status during pregnancy and parturition as significantly decreased level of MDA SOD and GSH-Px were observed in non-pregnant buffaloes compared to pregnant buffaloes on the day of parturition. The study indicated higher level of oxidative stress in buffaloes during parturition than non-pregnant buffaloes. The levels of these markers observed at 30 days after parturition was higher than non-pregnant buffaloes. This might be due to stress imposed by heavy lactation and incomplete involution of uterus in pregnant buffaloes. Keywords Oxidative stress Periparturient period Antioxidants buffaloes. INTRODUCTION Pregnancy and milk production although economic traits are physiological process but create a stressful condition as oxidative stress in the animal body. The oxidative stress is produced in multiple