The metabolic assay results indicated that vitrified tissue had viability similar to fresh controls. More recent study with tissue-engineered blood vessels using polyglycolic acid scaffold showed that ice formation in tissue-engineered blood vessels was negligible in the vitrified specimens but extensive ( + of vessel area) in the extracellular matrix of frozen specimens. The vitrified tissue had a viability similar to fresh controls and the contractility results for vitrified samples were of fresh controls but markedly reduced in the frozen samples ( for fresh controls) (Dahl et al., 2006). Vitrification is a feasible storage method for tissue-engineered blood vessel constructs, and their successful storage brings these.
