Almost a decade has passed since the last textbook on the science of cryobiology and the most common methods of cryopreservation was published [Fuller et al, 2007], to which we will refer as “the previous book” here and below. When it was published, it became a useful guide for both “seasoned” cryobiologists and those who had just started their journey to this fascinating science. However, there have been some serious tectonic shifts in cryobiology, which were perhaps not seen on the surface but may have a profound effect on both the future of cryobiology and on the development of new cryopreservation methods