The utility of primer pairs in SCoTs was advocated by Gorji et al. (2011). SCoT markers are generally reproducible, and it is suggested that primer length and annealing temperature are not the sole factors determining reproducibility (Gorji et al., 2011). They are dominant markers, however, while a number of co-dominant markers are also generated during amplification, and thus they could be used for genetic diversity analysis (Collard & Mackill, 2009b). This has been validated through study of genetic diversity among rice varieties (Collard & Mackill, 2009b). Further studies have utilized the technique to investigate genetic relationships in a number of plant species (Xiong et al., 2011; Murthy, 2011)
