The significance of flow cytometry can be summarized as the measure (-metry) of the optical properties of cells (cyto-) transported by a liquid sheath (flow) to a light source excitation (most often a laser) (Shapiro, 2003). FCM facilitates single cell analyses of both cell suspension, such as eukariotic and prokariotic cells, and “non cellular” suspension, such as microbeads, nuclei, mitochondria and chromosomes. A typical flow cytometer is formed by different units: the light source, the flow cell, the hydraulic fluidic system, several optical filters, a group of photodiodes or photomultiplier tubes and, finally, a data processing unit (Veal et al., 2000; Longobardi, 2001; Shapiro, 2003; Robinson, 2004; Diaz et.
