Chromatography was gained more importance initially after discovered in the preparative chromatography using large amount of the sample for the separation all components properly and collect each in their pure forms. Recently analytical chromatography found many application areas. The purpose of preparative Analytical chromatography is done normally with smaller amounts of material and is for measuring the relative proportions of analytes in a mixture after discovered some detectors which need very low amount of the analyte and have proper detection limits
