many new developments in infrared and Raman instrumentation, such as high sensitive detectors, charge-coupled devices (CCD) and array detectors, laser excitation sources, step-scan technique, photoacoustic detection, spectral depth profiling, light-fiber optics, mapping and imaging in the field of microscopy, time resolved and surface-enhanced methods - in order to mention a few of these significant improvements. Of course this progress in vibrational spectroscopy is closely connected with the enormous development in computer technique. These developments have created novel applications of IR and Raman spectroscopy in various scientific disciplines ranging from chemistry and physics to bioscience and medicine