Mutagenesis is a powerful method directed towards mutating genes or engineering genome for functional analysis, producing mouse models of human disease, and understanding molecular mechanism of biodiversity at different levels of biological organizations. Several insights have been revealed through studies using experimental models, or in silico, on natural mutations in general and site-specific mutagenesis in particular. However, unified text on recent trends in mutagenesis is poorly available within a single cover. Trust, this book would cater the need of readers who are looking for unified text on mutagenesis and its applications in research and teaching in the area of basic, agricultural or medical sciences