A reaction happens need the energy to vibrate the molecules and the reactant concentration enough. The energy here is often provided by heat. However, in living system, high temperature may harm the biological structure Truly that the concentration in living system is very low. So living organisms solve these problems by using enzyme | CHAPTER 2. ENZYMES INTERNATIONAL UNIVERSITY SCHOOL OF BIOTECHNOLOGY BIOCHEMISTRY Learning objectives 1. Comprehend how enzyme kinetics relates to the chemical kinetics presented in general chemistry courses. 2. Perceive how kinetic parameters are experimentally determined. 3. Learn the Michaelis–Menten equation and the meaning of KM and Vmax. 4. Understand the enzyme kinetics Brief Content WHAT IS ENZYME? CLASSIFICATION OF ENZYME 3. PROPERTIES OF ENZYME 4. ENZYME KINETICS 5. CATALYSIS (assignment) 6. ENZYME REGULATION (assignment) Detailed Content 1. WHAT IS ENZYME? 2. PROPERTIES OF ENZYME 3. CLASSIFICATION OF ENZYMES 4. ENZYME KINETICS Michaelis- Menten kinetics Lineweaver Burk plots Enzyme inhibition 5. CATALYSIS Catalytic mechanisms The role of cofactor in enzyme catalysis Effects of temperature and pH on enzyme-catalyzed reactions Detailed mechanisms of enzyme catalysis 6. ENZYME REGULATION Covalent modification Allosteric regulation 1. WHAT IS ENZYME? The most . | CHAPTER 2. ENZYMES INTERNATIONAL UNIVERSITY SCHOOL OF BIOTECHNOLOGY BIOCHEMISTRY Learning objectives 1. Comprehend how enzyme kinetics relates to the chemical kinetics presented in general chemistry courses. 2. Perceive how kinetic parameters are experimentally determined. 3. Learn the Michaelis–Menten equation and the meaning of KM and Vmax. 4. Understand the enzyme kinetics Brief Content WHAT IS ENZYME? CLASSIFICATION OF ENZYME 3. PROPERTIES OF ENZYME 4. ENZYME KINETICS 5. CATALYSIS (assignment) 6. ENZYME REGULATION (assignment) Detailed Content 1. WHAT IS ENZYME? 2. PROPERTIES OF ENZYME 3. CLASSIFICATION OF ENZYMES 4. ENZYME KINETICS Michaelis- Menten kinetics Lineweaver Burk plots Enzyme inhibition 5. CATALYSIS Catalytic mechanisms The role of cofactor in enzyme catalysis Effects of temperature and pH on enzyme-catalyzed reactions Detailed mechanisms of enzyme catalysis 6. ENZYME REGULATION Covalent modification Allosteric regulation 1. WHAT IS ENZYME? The most important functions of enzyme is their role as catalyst. All enzymes were considered to be protein but some examples of RNA molecules can also be catalyst. Living processes consists almost entirely of biochemical reactions. Without catalysts these reactions would not occur fast enough to sustain life. How a biochemical reaction can happen? A reaction happens need the energy to vibrate the molecules and the reactant concentration enough. The energy here is often provided by heat. However, in living system, high temperature may harm the biological structure Truly that the concentration in living system is very low. So living organisms solve these problems by using enzyme Which properties help enzyme a biocatalyst The rates of enzymatically catalyzed reactions are often phenomenally high The enzyme are highly specific to the reaction Enzyme has the complex structures so it can be regulated 2. PROPERTIES OF ENZYMES The active site Activation energy Cofactor 3. CLASSIFICATION OF .
