Put the right word in blanks in sentences from number 1 to number 20: explant, initiate, in vitro, non-dividing, differentiated, quiescent, nutrient, achive, differentiation, meristematic, redifferentiation. | In plant tissue culture, more often an explant( an excised piece of differentiated tissue or organ) is used to initiate their growth in culture. The non_dividing, differentiated, quiescent, cells of the explant when grown on a nutrent medium first undergo changes to achieve the meristematic state. The phenomenon of mature cells reverting to a meristematic state and forming undifferentiated callus tissue is termed as dedifferentiation. Since the multicellular explant comprises cells of diverse types, the callus derived will be heterogeneous. The ability of the component cells of the callus to differentiate into a whole plant or a plant organ is termed as redifferentiation. These two phenomena of dedifferentiation and redifferertiation are inherent in the capacity of a plant cell, and thus giving rise to a whole plant is described as cellular totipotency. Generally, a callus phase is involved before the cells can undergo redifferentiation leading to the regeneration of a whole plant. The dedifferentiated cells can rarely give rise to whole plants directly without an intermediate callus phase.
