QDs play an important role mainly in the imaging and as highly fluorescent probes for biological sensing that have better sensitivity, longer stability, good biocompatibility, and minimum invasiveness. The fluorescent properties of QDs arise from the fact, that their excitation states/band gaps are spatially confined, which results in physical and optical properties intermediate between compounds and single molecules. Depending on chemical composition and the size of the core which determines the quantum confinement, the emission peak can vary from UV to NIR wavelengths (400–1350 nm). In other words, the physical size of the band gap determines the photon’s emission wavelength: larger QDs having smaller band gaps emit red.