We established cell suspension culture on Panax vietnamensis and some attempts have been made to increase ginsenoside yield of ginseng cell culture through manipulation various culture factors and process variable. Half and full strength MS medium were found to be equally suitable for both biomass as well as ginsenoside production. The biomass production and ginsenoside yield were obtained g/L DW and mg/g DW, respectively. The effect of initial sucrose concentrations were also investigated in suspension cultures of P. vietnamensis for biomass and production of ginseng saponin (secondary metabolite). The final dry cell weight was increased from to g/L with an increase of initial.