We report here the protocol for Zedoary (Curcuma zedoaria Roscoe) callus and cell suspension cultures. The MS medium supplemented with 2% sucrose, mg/l 2,4-D and mg/l BA was effective for callus induction from in vitro leaf-base explants of Zedoary. During subcultures, secondary proliferated calli were subsequently produced from initial induced calli on the MS medium with mg/l and mg/l BA. These calli were light yellow in color, compact and friable.