The gene 4CL1 was isolated from Chinese red pine (Pinus massoniana Lamb) and ligated into vector pPTN289 to perform transformation vector pPTN289-4CL1. This construction was transformed into Agrobacterium tumefaciens strain C58, and then transformed into Chinaberrytree (Melia azedarach L.). The transgenic Chinaberrytree was screened on selection medium (MS + 6-BA + 1mg/l vitamine B5 + 30g/l sucrose + 8g/l agar + 500mg/l Cefotaxime + 1mg/l PPT) and then extracted total DNA and tested the existence of interested gene using PCR method. .
