There has been some concern that the use of the endocervical brush can result in the appearance of a much greater number of endocervical cells in a smear and that their arrangement in large sheets might mimic malignancy. To avoid this problem clinicians should inform the laboratory when an endocervical brush is used for collecting the smear. Preparation of Smear: After smear collection, the cellular sample is evenly smeared on to the centre of the non-frosted area of the glass slide, by rotating both sides of the scrape end of the spatula in multiple clockwise swirls in contact with the slide and fixing it immediately Excessively thin.
