Creation of a vacuum and obtaining the material: Suction is applied after entering the lesion and while maintaining the suction, needle is moved vigorously back and forth in a sawing or cutting motion, changing the direction a few times, ensuring that the needle is inside the mass throughout; the whole procedure taking only 4-8 seconds. Do not rotate the needle or pump the plunger in the syringe in and out. Purpose of suction is to pull the tissue against the cutting edge of the needle and to pull the dislodged tissue fragments and cells into the lumen of the needle. Material is procured by cutting motion.
