Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. Mucins are a major component of the interface between the external world and mucosal tissues, where they provide lubrication, hydration, and a biological and physical barrier to potential toxins, particles, and pathogens. Mucins provide many challenges to researchers due to their large size, complex biochemical nature, and the viscous gels that they form when secreted. Overcoming these challenges is centrally important to a full understanding of mucosal biology and the contribution of mucins to normal human physiology and disease. In this volume of the Methods in Molecular Biology series, we have highlighted the.
