PCR-RFLP analysis of beta-lactoglobulin gene locus was carried out on 110 DNA samples of Murrah buffaloes in the present study. A 262 bp fragment enclosing from exon IV to intron IV in b-lg gene was amplified with specific primers. All the 110 DNA samples resulted in 262 bp product on amplification. The PCR products were subjected for digestion with Pst1,EcoRI, HindIII and Hae III enzyme. PCR products were not digested by PstI, EcoRI and HindIII. PCR products when digested with HaeIII enzyme resulted in monomorphic banding pattern in all the samples. Sequencing of PCR products also revealed no polymorphism ( Gen Bank DQ340204 ) The.
