In most agricultural settings, commercial fertilizer provides only one source of N used for crop production. Animal manure, biological N fixation, mineralization from soil organic N, and deposition of N from the atmosphere can also contribute to soil fertility and surface water contamination. Because there are multiple sources and sinks of N in the soil, the relationship between N fertilizer application rate and nitrogen loss in drainage water is not always consistent across locations and across studies. If denitrification and plant and microbial uptake of N are large, nitrate concentrations in subsurface drainage may be low in spite of high fertilizer N inputs. If mineralization of soil organic matter is large, nitrogen.