Laccase-encoding sequences sharing 65–71% identity were shuffledin vivo by homeologous recombination. Yeast efficiently repaired linearized plas-mids containingclac1, clac2 orclac5 Trametes sp. C30 cDNAs using a clac3 PCR fragment. From transformants secreting active variants, three chimeric laccases (LAC131, LAC232 and LAC535), each resulting from double crossovers, were purified, and their apparent kinetic parameters were determined using 2,2¢-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) and syringaldazine (SGZ) as substrates. .
