In living organisms, genes encoding proteins that contain flavins as a pros-thetic group constitute approximately 2–3% of the total. The fluorescence of flavin cofactors in these proteins is a property that is widely employed for biochemical characterisation. Here, we present a modified Thermofluor approach called ThermoFAD (Thermofluor -adapted flavin ad hoc detec-tion system), which simplifies identification of optimal purification and storage conditions as well as high-affinity ligands.
