A procedure has been developed for the isolation of cell walls from the hyphae of the causal agent for barley leaf scald,Rhynchosporium secalis (Oudem) . Davis. Based primarily on monosaccharide linkage analysis, but also on the limited use of linkage-specific glucan hydrolases and solvent fractionation, the walls consist predominantly of (1,3⁄1,6)-b-d-glucans