The genome of the mesophilic Gram-negative bacteriumBurkholderia thai-landensis contains an open reading frame (. theBth_I1158gene) that has been annotated as a putative ribokinase and PFK-B family member. Nota-bly, although the deduced amino acid sequence of the gene showed only 29% similarity to the recently identified nucleoside kinase from hyperthermophilic archaeaMethanocaldococcus jannaschii, 15 of 17 residues reportedly involved in the catalytic activity of M.