The human prion protein (PrP) is a glycoprotein with a glycosylphosphat-idylinositol (GPI) anchor at its C-terminus. Here we report alternative splic-ing within exon 2 of the PrPgene (PRNP) in the human glioblastoma cell line T98G. The open reading frame of the alternatively spliced mRNA lacked the GPI anchor signal sequence and encoded a 230 amino acid polypeptide.