TheVibriometalloprotease PrtV was purified from the culture supernatant of aVibrio choleraederivative that is deficient in several other secreted pep-tidases, including the otherwise abundant hemagglutinin⁄protease HapA. The PrtV is synthesized as a 102 kDa protein, but undergoes several N- and C-terminal processing steps duringV. choleraeenvelope transloca-tion and prolonged incubation.