The hexose transporter family, which mediates facilitated uptake in mammalian cells, consists of more than 10 members containing 12 mem-brane-spanning segments with a single N-glycosylation site. We previously demonstrated that glucose transporter 1 is organized into a raft-like deter-gent-resistant membrane domain but that glucose transporter 3 distributes to fluid membrane domains in nonpolarized mammalian cells.