Two mutants ofChlamydomonas reinhardtii, mf1andmf2, characterized by a marked reduction in their phosphatidyl-glycerol content together with a complete loss in itsD 3 -trans hexadecenoic acid-containing form, also lost photosystemII (PSII) activity. Genetic analysis of crosses betweenmf2and wild-type strains shows a strict cosegregationof thePSII and lipid deficiencies, while phenotypic analysis of phototrophic revertant strains suggests that one single nuclear mutation is responsible for the pleiotropic phenotype of the mutants
