We have cloned and determined the nucleotide sequence of the cDNAcoding for a cellular retinol-bindingprotein type I (CRBPI)from zebrafish. The deduced amino acid sequence of the zebrafish CRBPI showed highest sequence identity ( 59%)to the mammalian CRBPIs of the intracellular lipid-binding protein (iLBP)multigene family. Phylogenetic analysis clustered the zebrafish CRBPI to the CRBPI clade. The zebrafish CRBPI gene (rbp1)and CRBPII gene (rbp2) both consist of four exons separated by three introns, iden-tical to all otheriLBP genes in vertebrates