The binding characteristics of a series of PPARcligands (GW9662, GI 262570, cis-parinaric acid, 15-deoxy-D 12,14 -prostaglandin J2 , LY171883, indomethacin, linoleic acid, palmitic acid and troglitazone) to human PPARcligand binding domain have been investigated for the first time by using surface plasmon resonance biosensor technology, CD spectroscopyandmoleculardockingsimulation.