Phosphorylationof the transit peptideof several chloroplast-targeted proteins enables the binding of 14-3-3 proteins. The complex that forms, together with Hsp70, has been demonstrated to be an intermediate in the chloroplast pro-tein import pathwayin vitro[May, T. &Soll, J. (2000)Plant Cell12, 53–63]. In this paper we report that mutagenesis (in order to remove the phosphorylation site) of the transit peptide of the small subunit of ribulose bisphosphate carb-oxylase/oxygenase did not affect its ability to target green fluorescent protein to chloroplastsin vivo
