Phosphoglyceratemutase (PGAM) activity inpromastigotes of theprotozoanparasiteLeishmaniamexicanais foundonly in the cytosol. It corresponds to a cofactor-independent PGAM as it is not stimulated by 2,3-bisphosphoglycerate and is susceptible to EDTA and resistant to vanadate. We have cloned and sequenced the gene and developed a convenient bacterial expression system and a high-yield purification protocol. Kinetic properties of the bacterially produced protein have been determined (3-phosphoglycer-ate:Km¼ ± mM, kcat ¼434 ± 54 s )1 ; 2-phos-phoglycerate:Km¼ ± mM,kcat ¼199 ± 24 s )1 ). The activity is inhibited by phosphate but is resistant to Cl – andSO4 2– . .
