Enzyme catalysis in low water containing organic solvents is finding an increasing number of applications in diverse review focuses on some aspects which have not been reviewed strategies for obtaining higher activity and stability in such media are this context, the damaging role of lyophilization and the means of overcoming such effects are and microwave assistance are two emerging approa-ches for enhancing reaction rates in low water media. Control of water activity and medium engineering are two crucial approaches in optimization of catalytic behaviour in nonaqueous enzymology