Phospholipase D (PLD) plays amajor role in the activation of the neutrophil respiratory burst. However, the repertoire of PLD isoforms present in these primary cells, the precise mechanism of activation, andthe impact of cell priming on PLD activity and localization remain poorly defined. RT-PCR analysis showedthat both PLD1 andPLD2 iso-forms are expressedin human neutrophils, with PLD1 expressedat a higher level. Endogenous PLD1 was detected by immunoprecipitation andWestern blotting, andwas predominantly membrane-associated under control and primed/stimulated conditions
