We reportedpreviously a direct correlationbetween reduced soybean agglutinin binding to 63- and 68-kDa midgut gly-coproteins and resistance to Cry1Ac toxin from Bacillus thuringiensisin the tobacco budworm (Heliothis virescens). In the present work we describe the identification of the 68-kDaglycoproteinas amembrane-bound formof alkaline phosphatase we term HvALP. Lectin blot analysis of HvALP revealed the existence of N-linked oligosaccharides containing terminal N-acetylgalactosamine required for [ 125 I]Cry1Ac binding in ligand blots. .
