The kinetic data on sugarcane (Saccharumspp. hybrids) sucrose synthase (SuSy, UDP-glucose: D-fructose 2-a-D-glucosyltransferase, EC ) are limited. We character-ized kinetically a SuSy activity partially purified from sugarcane variety N19 leaf roll tissue. Primary plot analysis and product inhibition studies showed that a compulsory order ternary complex mechanism is followed, with UDP binding first and UDP-glucose dissociating last from the enzyme.