In this report, we have focused our attention on identifying intracellular mammalian proteins that bind S100A12 in a Ca 2+ -dependent manner. Using S100A12 affinity chroma-tography, we have identified cytosolic NADP + -dependent isocitrate dehydrogenase (IDH), fructose-1,6-bisphosphate aldolase A (aldolase), glyceraldehyde-3-phosphate dehy-drogenese (GAPDH), annexin V, S100A9, and S100A12 itself as S100A12-binding proteins. Immunoprecipitation experiments indicated the formation of stable complexes between S100A12 and IDH, aldolase, GAPDH, annexin V and S100A9in vivo
