Mitochondrial biogenesis is a complex and highly regulated process that requires the controlled expression of hundreds of genes encoded in two separated genomes, namely the nuclear and mitochondrial genomes. To identify regulatory proteins involved in the transcriptional control of key nuc-lear-encoded mitochondrial genes, we have performed a detailed analysis of the promoter region of theasubunit of the Drosophila melanogasterF1F0 ATP synthase complex. Using transient transfection assays, we have identified a 56 bpcis-acting proximal regulatory region that contains binding sites for the GAGA factor and the alcohol dehy-drogenase distal factor 1
