1,5-Diamino-2-pentyne (DAPY) was found to be a weak substrateof grasspea (Lathyrus sativus,GPAO) andsainfoin (Onobrychis viciifolia, OVAO) amine oxidases. Prolonged incubations, however, resulted in irreversible inhibition of both enzymes. For GPAOandOVAO, rates of inactivation of – min )1 were determined, the apparentKI values (half-maximal inactivation) were of the order of 10 )5 M. DAPY was found to be amechanism-based inhibitor of the enzymes because the substrate cadaverine significantly pre-vented irreversible inhibition. .
