The cellobiohydrolase Pc_Cel7D is the major cellulase produced by the white-rot fungusPhanerochaete chrysosporium, constituting 10% of the total secreted protein in liquid culture on cellulose. The enzyme is classified into family 7 of the glycoside hydrolases and, like other family members, catalyses cellulose hydrolysis with net retention of the anomeric carbon configuration.