The structure of the complex between the minor groove binder netropsin and d(GGCCAATTGG) was determined via single-crystal X-ray tech-niques. The structure was refined to completion , result-ing in a residual R-factor of (including 68 water molecules). Using crystal engineering and cryocooling techniques, the resolution could be enhanced to A ˚ , resulting in an unambiguous determination of the drug conformation and orientation.