The heme of cytochrome P460 ofNitrosomonas europaea, which is covalently crosslinked to two cysteines of the polypeptideaswithallc-type cytochromes, has anadditional novel covalent crosslink to lysine 70 of the polypeptide [Arciero,. (1997), 457– 460]. The protein can catalyze the oxidation of hydroxyl-amine. The gene for this protein,cyp,wasexpressedin Pseudomonas aeruginosastrain PAOlacI, resulting in for-mation of a holo-cytochrome P460 which closely resembled native cytochrome P460 purified fromN. .
