The cardiotoxin (cytotoxin II, or CTII) isolated from cobra snake (Naja oxiana) venom is a 60-residue basic membrane-activeprotein featuring three-fingerbetasheet possible modes of CTII/membrane interaction 31 P- and 1 H-NMR spectroscopy was used to study binding of the toxin and its effect onto multilamellar vesicles (MLV) composed of either zwitterionic or anionic phospholipid, dipalmitoylglycerophosphocholine (Pam2 Gro-PCho) or dipalmitoylglycerophosphoglycerol (Pam2 Gro-PGro), res-pectively. .