The targeting of proteins to particular subcellular sites is an important principle of the functional organization of cells at themolecular level. In turn, knowledge about the subcellular localizationof aprotein is acharacteristic thatmayprovidea hint as to the function of the protein. The combination of classic biochemical fractionation techniques for the enrich-ment of particular subcellular structures with the large-scale identification of proteins by mass spectrometry and bio-informatics provides a powerful strategy that interfaces cell biology andproteomics, andthus is termed subcellular proteomics . .
