It is known that phosphatidylinositol (PtdIns) contains abundant arachidonate and is composed mainly of 1-stea-royl-2-arachidonoyl species in mammals. We investigated if this characteristic of PtdIns applies to the PtdIns from yellowtail (Seriolaquinqueradiata),amarinefish. Incommon with phosphatidylcholine (PtdCho),phosphatidylethanol-amine (PtdEtn) andphosphatidylserine (PtdSer) frombrain, heart,liver,spleen,kidney and ovary,the predominant polyunsaturated fatty acid was docosahexaenoic acid,and levels of arachidonic acid were less than (PtdCho), (PtdEtn) (PtdSer) in these tissues