In this article, we report the results of an analysis of the glycolytic enzyme enolase (2-phospho-D-glycerate hydro-lase) of Trypanosoma brucei. Enolase activity was detected in both bloodstream-form and procyclic insect-stage try-panosomes, although a lower specific activity was found in the cultured procyclic homogenate. Subcellular localization analysis showed that the enzyme is only pre-sent in the cytosol.