We have determined the cDNA nucleotide sequence, deduced the amino acid sequence and defined the gene structure for the cellular heart-type (H-FABP) or fatty acid-binding protein 3 (FABP3) from zebrafish. The zebrafish FABP3 exhibited the greatest amino acid sequence identity to fish andmammalian heart-type FABPs. 3¢RACE and 5¢ RLM-RACE mapped two alternative polyadenylation sites and three transcription start sites, respectively.