The characterization of the gene encodingLeishmania donovaniphosphofructokinase (PFK) and the biochemical properties of the expressedenzyme are . donovani has a singlePFKgene copyperhaploidgenome that encodes a polypeptide with a deducedmolecular mass of 53 988 and a pI of . The predicted amino acid sequence contains a C-terminal tripeptide that conforms to an established signal for glycosome targeting.