Equilibrium studies on the acid included denaturation of stem bromelain (EC ) were performed by CD spectroscopy, ¯uorescence emission spectroscopy and binding of the hydrophobic dye, 1-anilino 8-naphthalene sulfonic acid (ANS). At pH , stembromelain lacks awell de®ned tertiary structure as seen by ¯uorescence and showretentionof some native like secondary structure at pH . Interdisciplinary Biotechnology Unit, Aligarh Muslim University, India Equilibrium studies on the acid included denaturation of stem bromelain (EC ) were performed by CD spectroscopy, ¯uorescence emission spectroscopy and binding of the hydrophobic dye, 1-anilino 8-naphthalene sulfonic acid (ANS). At pH , stem bromelain lacks a well de®ned tertiary structure as seen by.
